Antitumor effects of a polypeptide isolated from Tegillarca granosa linnaeus and the related molecular mechanism

ABSTRACT

This study is to investigate the anticancer effects and mechanisms of Tegillarca granosa Linnaeus-1 [TG-1] on renal carcinoma OS-RC-2 cells in vitro. The proliferation of OS-RC-2 cells was evaluated under various concentrations of TG-1 using MTT assay. The apoptosis of OS-RC-2 cells was analyzed using acridine orange/ethidium bromide staining. And the cell cycle distribution of OS-RC-2 cells was detected by flow cytometry. In addition, the expression level of Ki67 mRNA was examined by RT-PCR and level of casepase-3 was examined by Western blot analysis. TG-1 incubation significantly inhibited the proliferation of renal carcinoma OS-RC-2 cells and arrested cells at G[0]/G[1] phase [P <0.05]. And TG-1 also significantly inhibited the expression of Ki67 mRNA [P<0.05]. Additionally, TG- 1 significantly promoted apoptosis and the expression of caspase-3 in cells [P<0.05]. Moreover, the optimal effects of TG-1 was achieved at the concentration of 100 mg/L The results indicate that TG-1 has antitumor effects on renal carcinoma OS-RC-2 cells and that the underlying mechanisms may be acted through inhibiting proliferation and Ki67 mRNA expression, and promoting apoptosis and caspase-3 expression.