In-vitro evaluation of cytotoxic and apoptogenic properties of sophora pachycarpa

ABSTRACT

Sophora pachycarpa Schrenk ex C.A.Mey. belongs to the family Fabaceae. Some species of the genus Sophora have shown to possess anti-proliferative and apoptosis-inducing activities in cancer cells. However, there is no available information addressing this effect in S. pachycarpa. Here, we investigated the cytotoxic effects of methanol extract and different fractions obtained from S. pachycarpa root on different cancer cell lines including A549, HeLa, HL-60, MCF-7, and PC3 cell lines and leukocytes as non-malignant cells. Apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry [sub-Gl peak]. S. pachycarpa inhibited the growth of malignant cells in a dose-dependent manner. CH[2]C1[2] and EtOAc fractions showed the lowest IC[50] values ranging from 6 to 50 microg/mL in various cancer cell lines. HeLa cells as the most sensitive cells were chosen for further mechanistic studies. The sub-Gl peak in flow cytometry histogram of S. pachycarpa treated HeLa cells indicates apoptotic cell death in S. pachycarpa-'mduced toxicity. In conclusion, S. pachycarpa exerts cytotoxic effects in different cancer cell lines in which apoptosis plays an important role. Thus, S. pachycarpa could be considered as a potential chemotherapeutic agent in cancer treatment