[Comparison of the expression level of P53 and proliferating cell nuclear antigen [PCNA] genes in the inflamed and non-inflamed dentigerous cysts]

ABSTRACT

The role of P53 and proliferating cell nuclear antigen [PCNA] genes in inflamed and non-inflamed odontogenic cysts is related to cell proliferation but it is unclear. The aim of this study was immunohistochemical evaluation of P53 and PCNA expression in inflamed and non-inflamed dentigerous cysts. In the present study, 23 dentigerous cysts [12 inflamed and 11 non-inflamed] were surveyed. Three microns slices were got from paraffined blocks and stained with P53 and PCNA antibodies by immunohistochemistry. Slides were seen by oral pathologist with optical microscope [Olympus BX41] in x400 magnification and scored by Allred scoring. Data were analyzed by Mann-Whitney test via SPSS software. Percent of stained cells in inflamed compared non-inflamed dentigerous cyst did not show any significant difference for expression level of P53 and PCNA in basal and suprabasal layers [P> 0.05]. It seems that inflammation does not affect on expression level of P53 and PCNA in dentigerous cyst