Gene expression and pulmonary toxicity of chitosan-graft-polyethylenimine as aerosol gene carrier
IJPR-Iranian Journal of Pharmaceutical Research. 2013; 12 (2): 281-286
in English
| IMEMR
| ID: emr-142647
ABSTRACT
Chitosan-graft-polyethylenimine [CHI-g-PEI] copolymer has been used for the improvement of low transfection efficiency of chitosan. The present study aims to test the pulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposed to aerosol containing green-fluorescent protein [GFP]-polyethylenimine [PEI] or GFP-CHI-g-PEI complexes for 30 min during the development of our nose-only exposure chamber [NOEC] system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancement of the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonary toxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol gene delivery to animals, and enhanced efficiency was achieved through our aerosol gene delivery system. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosol gene therapy
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Gene Expression
/
Gene Transfer Techniques
/
Chitosan
/
Green Fluorescent Proteins
/
Mice
Language:
English
Journal:
Iran. J. Pharm. Res.
Year:
2013
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