Potentiating effects of Lactuca sativa on pentobarbital-induced sleep
IJPR-Iranian Journal of Pharmaceutical Research. 2013; 12 (2): 401-406
in English
| IMEMR
| ID: emr-142661
ABSTRACT
Traditionally, Lactuca sativa [lettuce] has been recommended for its hypnotic property. The present study was planned to investigate sleep-prolonging effect of this plant. The hydro-alcoholic extract [HAE] of lettuce and its water fraction [WF], ethyl acetate fraction [EAF], and n-butanol fraction [NBF] were administrated [IP] to mice 30 min before the pentobarbital injection. Moreover, both in-vivo and in-vitro toxicity of the extracts were determined. The quality of HAE and NBF was also evaluated using HPLC fingerprint. The HAE prolonged the pentobarbital-induced sleep duration at dose of 400 mg/Kg. The NBF was the only fraction which could increase the sleep duration and decrease sleep latency. The effects of NBF were comparable to those of induced by diazepam. The LD[50]-value for HAE was found to be 4.8 g/Kg. No neurotoxic effect was observed either by HAE or by its fractions in cultured PC12 neuron-like cells. The results suggest that lettuce potentiates pentobarbital hypnosis without major toxic effect. The main component[s] responsible for this effect is most likely to be non-polar agent[s] which found in NBF of this plant
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Pentobarbital
/
Sleep
/
Plant Extracts
/
PC12 Cells
/
Hypnotics and Sedatives
/
Mice
Limits:
Animals
Language:
English
Journal:
Iran. J. Pharm. Res.
Year:
2013
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