Effect of thermal stress on MICA/B induction in a human liposarcoma cell line
IJI-Iranian Journal of Immunology. 2013; 10 (1): 31-39
in English
| IMEMR
| ID: emr-142675
ABSTRACT
A possible mechanism by which hyperthermia enhances tumor immunogenicity is the induction of NKG2D ligands on tumor cells. Although the expression of MHC class I chain-related protein A and B [MICA/B] has previously been reported in different carcinomas, there is no information about MICA/B expression in liposarcomas. To investigate MICA/B induction in a human liposarcoma cell line [SW-872] after thermotherapy. SW-872 and HeLa cell lines were subjected to thermal stress for 1 h at 42, 44 and 46[degree sign]C, and after 2, 4 and 6 h of incubation at 37[degree sign]C, MICA/B expression was assessed at the mRNA and protein levels. Despite high levels of MICA/B transcripts in SW-872 cells at baseline, the expression of these genes decreased significantly at both the mRNA and protein levels after almost all thermal treatments. Our data conclude that thermotherapy under 42-46[degree sign]C had no effect on MICA/B induction on SW-872 liposarcoma cell line but the effects of fever-range temperatures remain to be tested on this cell line
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Killer Cells, Natural
/
Cell Line, Tumor
/
NK Cell Lectin-Like Receptor Subfamily K
/
Hyperthermia, Induced
Language:
English
Journal:
Iran. J. Immunol.
Year:
2013
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