[Spectroscopic method for the determination of dorzolamide hydrochloride and timolol maleate in it dosage form/ eye drops]

ABSTRACT

Simple, sensitive and specific method for the simultaneous determination of Dorzolamid HCI and Timolol maleate in its dosage form /Eye drops/. The method depended on dilution of 1 volume of the Eye drops 100 time with distillers water, then 10 ml from the resulting solution to 50 ml of water and measure the absorbance at 258 nm and 295 nm against water. The proposed UV-method was successfully applied to the determination of sibotramin in commercial product Timodors [registered sign] [diamondpharm]. The percentage recoveries and the standard division [n=10] were 98.8 +/- 0.92%. For Dorzolamid and 98.1 +/- 0.95 for timolol. The suggested method was compared with HPLC method while the result was conform with the standard method according to statistic Value for F and T tests