Analysis of polymorphism of MHC class II BuLA DRB3 exon 2 gene in north west Iranian populations of the water buffalo [Bubalus bubalis] through PCR-SSCP
INTJVR-International Journal of Veterinary Research. 2010; 4 (4): 265-268
in English
| IMEMR
| ID: emr-143697
ABSTRACT
The DRB3 gene is a highly polymorphic major histocompatibility complex [MHC] class II gene and plays an important role in variability of immune responsiveness and disease resistance. In the present study, the MHC class II DRB3 gene in water buffalo [Bubalus bubalis] populations from Northwest regions of Iran was investigated through PCR-SSCP. Genomic DNA was extracted from whole blood samples collected from 50 buffaloes. A 284 bp segment of exon 2 of BuLa-DRB3 was amplified by standard PCR, using locus-specific primers. The PCR products were subjected to a non-denaturing gel electrophoresis. A number of 11 different SSCP patterns indicating allelic variation were identified. The three most frequent patterns 1,4 and 10 accounted for 58% of the total patterns. Results indicated that exon 2 of the BuLA-DRB3 gene is highly polymorphic among the examined animals
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymorphism, Genetic
/
Buffaloes
/
Genes, MHC Class I
/
HLA-DR Antigens
/
Polymerase Chain Reaction
/
Polymorphism, Single-Stranded Conformational
/
Major Histocompatibility Complex
Limits:
Animals
Language:
English
Journal:
Int. J. Vet. Res.
Year:
2010
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