[Designing of human cartilage tissue, by differentiation of adipose-derived stem cells with BMP-6 in alginate scaffold]
Journal of Iranian Anatomical Sciences. 2010; 8 (31): 117-127
in Persian
| IMEMR
| ID: emr-143869
ABSTRACT
In the present study the effect of BMP-6 was investigated on chondrogenesis of adipose-derived stem cells. Mesenchymal stem cells derived from subcutaneous adipose tissue were cultured on alginate scaffold to induce chondrogenesis in experimental group, with chondrogenic medium having BMP-6 growth factor for 3weeks. In control group medium without BMP-6 was applied. The harvested constructs were examined with immunohistochemical and RT-PCR methods for assessment of cartilage-specific characteristics. The results of immunohistochemical method revealed the presence of typical cartilage extracellular matrix components such as type II collagen and aggrecan in constructs induced by BMP-6 growth factor on alginate scaffold. In addition evaluation of the results of RT-PCR analysis confirmed the expression of cartilage- specific genes, such as type II collagen and aggrecan, in the differentiated cells under the influence of growth factor BMP-6. It can be concluded that BMP-6 promotes chondrogenesis of ADSC in 3-D and adipose-derived stem cells could be used for cartilage tissue engineering
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Stem Cells
/
Cartilage
/
Adipose Tissue
/
Alginates
/
Tissue Scaffolds
/
Bone Morphogenetic Protein 6
Language:
Persian
Journal:
J. Iran. Anat. Sci.
Year:
2010
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