clot-lysis effect of selective alpha[1]-adrenoceptor antagonist in vitro model associated with high peroxynitrite level

ABSTRACT

Alpha[1]-adrenoceptor blocking agents showed several effects beyond their action on the vascular smooth muscles. They improve the lipid profile and inhibit the aggregation of blood platelets. To investigate the clot-lysis effect of selective alpha[1]-adrenoceptor antagonists and its relation to peroxynitrite level in vitro experimental model. Venous blood samples obtained from ten healthy subjects. To each pre-weighed clot, 100 micro L of either distilled water as a negative control, prazosin [10 micro g], terazosin [20 micro g] and alfuzosin [25 micro g] were added. Peroxynitrite level was measured in sera and sangious fluid that formed after clot-lysis. Prazosin, terazosin and alfuzosinjn order, significantly reduced the clot weight up to 3.7%. Peroxynitrite level in sangious fluids was higher in treated groups than that of negative control or sera levels. Alpha[1]-adrenoceptor antagonists induced clot-lysis effect. This effect is associated with generation peroxynitrite