INTJVR-International Journal of Veterinary Research. 2010; 4 (3): 159-161
in English
| IMEMR
| ID: emr-145040
ABSTRACT
Exogenous nitric oxide donors such as DETA NONOate, spontaneously release nitric oxide. This study aimed to investigate the effect of DETA NONOate as a nitric oxide releasing drug on the rate of collagen synthesis during the impaired wound healing in a rat model of diabetes. Twelve male Sprague-Dawley rats were transferred into separate metabolic cages. Nine days before wounding, the rats were injected intraperitoneally with streptozotocin [STZ; 55 mg/kg body weight in citrate buffer 0.1 mol/L, pH 4.5] to induce diabetes. The dorsal surface of each rat was properly shaved and a full thickness dermal wound was made. The test group [n=6] was treated with 100 microM DETA NONOate in phosphate buffer while the control wounds [n=6] received sterile saline [PBS] only on the same day as wounding and every three days for one week. After the skin incision, polyvinyl alcohol [PVA] sponges were implanted subcutaneously on the dorsal of each animal under sterile conditions for the collection of wound fluid. Electrophoresis [current 20 mA] was performed on the wound fluid. The gel was stained with Coomassie blue G-250, destained, and photographed. DETA NONOate treatment increased the rate of collagen synthesis in the diabetic test group compared to the control group. The nitric oxide donor, DETA NONOate, may represent a potential treatment for impaired wound healing in diabetes by increasing the collagen synthesis at the wound site
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Wound Healing
/
Collagen
/
Rats, Sprague-Dawley
/
Nitric Oxide Donors
/
Models, Animal
/
Diabetes Mellitus, Experimental
Limits:
Animals
Language:
English
Journal:
Int. J. Vet. Res.
Year:
2010
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