Pathomorphological effects of alloxan induced acute hypoglycaemia in rabbits

ABSTRACT

Alloxan is one of the frequently used beta-cytotoxic agents for the induction of Type-1 diabetes mellitus in animal models and is the drug of choice in rabbits. Its beta-cytotoxic action results in a sudden release of insulin leading to severe hypoglycaemia and even mortality if glucose therapy is not given. In the present investigation the pathological effects of alloxan induced acute hypoglycaemia were studied in rabbits. New Zealand White rabbits, 1-1.5 kg body weight, were administered alloxan@100 mg/kg b.w., as a single intravenous dose. Blood glucose levels were monitored [0 h, 20 min, 1 h, and then hourly up to 5 h] and clinical signs noted. Rabbits dead due to hypoglycaemia were necropsied and histopathology performed. Severe histopathological changes were observed especially in the brain [neuronal degeneration and necrosis], kidneys [nephrosis, nephritis] and liver [hepatosis, hepatitis] and also, other organs. Histopathological observation of beta-cytolysis was suggestive that the drug induced hypoglycaemia is insulin mediated. It was concluded that acute hypoglycaemia causes severe pathological changes and the alloxan induced immediate hypoglycaemia if not managed in time, might exacerbate the pathological effects of hyperglycaemia in the induced diabetic models