Hodgkin's disease: cytochemical studies of deoxyribonucleic acid [DNA] and glucose 6-phosphate dehydrogenase [C6PD] after cyclopho-sphamide [endoxan] treatment

ABSTRACT

Deoxyribonucleic acid [DNA] and glucose 6- phosphate dehydrcgenase [G6PD] enzyme were studied in human normal lymph node and Hodgk in's disease cells. It has been observed that DNA was present in chromatin materials, whereas [G6P D] was present as granules distributed uniformaly in the cytoplasm. Under the effect of endoxan, a decreased activity of DNA and G6PD has been noticed. This could be explained on the basis that alkylation of phosphate groups was the main point of attack on the DNA which result in the breakdown of the DNA molecule