Role of trichinella spiralis adult and larval antigen in immunomodulation of nitric oxide [NO] expression in intestinal and muscular phase of trichinellosis

ABSTRACT

Nitric oxide [NO] mediates regulatory events that play key roles in cellular function. NO is produced through the action of nitric oxide synthase [iNOS] it is considered to be the main factor able to kill or at least to suppress various pathogens, including intracellular parasites as Plasmodium, Leishmania major, Trypanosoma cruzi and Toxoplasma gondii. The aim of this study is to investigate the role of. spiralis Adult Antigen [TAA] and Larval Antigen [TLA] on the expression of iNOS in both phases of infection at different postinfection intervals using specific anti-iNOS antibody. Immunohistochemistry was performed among muscle and intestinal parts excised from three groups of laboratory mice [along with control groups] at different post infection intervals Group 1, [G[1]] T. Spiralis infected mice and none immunized, Group 2, [G[2]] T. spiralis infected mice and immunized with [TLA] and Group 3, [G[3]] T spiralis infected mice and immunized with [TAA]. NO synthase immunoreactivity was detected by using peroxidase-labelled streptavidin-biotin technique and the CD3 activity [anti T-cells] was detected using Clone FC3/188A Ab The study results showed that Trichinella antigen [TLA and TAA] had modulated the expression of NO in each phase of infection. TLA was more potent than TAA in activation of NO during the muscular phase and decreasing its activity during the intestinal phase. By the inverse effect of these antigens on [NO], the integrity of the intestinal and muscular tissues had been maintained