effect of curing cycle of denture base [PMMA] on microcolonization of candida albicans

ABSTRACT

The purpose of the study was to assess the growth of Candida Albicans on Polymethylmethacrylate [PMMA] as a denture base, using various curing cycles. Total 200 samples of denture bases were prepared divided into five categories, cut into 2x2 cm, with the thickness of 1.0 mm for the analysis. 160 out of them were further divided into 4 groups for heat cure water bath polymerization. They were assessed on different temperatures i.e. 45[degree sign] c, 100 [degree sign] c 70[degree sign] c, and 70 [degree sign] c for the time span of 45, 60, 420 and 540 min respectively. The specimens of the 5th group were cured chemically. Every specimen of all the five groups was kept in the bottles along with the food stuff for 12, 24, 168, and 336 hrs. The specimens were then mounted on the glass slides to examine under the light microscope for the analysis of Candida Albicans. Results showed that the growth of Candida Albicans observed to be reduced in heat cure polymerization as compared to the ones, cured chemically. Thus concluded that among all the sample types, the group D cured at the temperature of 70 [degree sign] c for 540 min have shown the minimum growth of Candida Albicans