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Comparison between RT-PCR and viral solation for detection OFH9 subtype of avian influenza viruses
Iranian Journal of Veterinary Research. 2008; 63 (1): 17-22
in Persian | IMEMR | ID: emr-146236
ABSTRACT
During 2004-2006, a total number of 22 tissue samples obtained from poultry flocks suspected to respiratory diseases submitted to avian virology laboratory of faculty of veterinary medicine, were prepared for avian influenza virus [AIV] isolation in embryonated chicken eggs, according to the standard method. RT-PCR was established on tissue samples using specific primers for H9 gene. 3.5 Amplified PCR products were 435 bp in length. Analytical sensitivity of the RT-PCR was 10 EID50 and sensitivity, specificity and correlation rate compared with virus isolation, were 100%, 94% and 95%, respectively. The results showed that the RT-PCR assay using H9 gene specific primers, directly on tissue samples, could be used in rapid detection and subtyping of H9 AIVs as a useful alternative to virus isolation assay
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Index: IMEMR (Eastern Mediterranean) Main subject: Sensitivity and Specificity / Reverse Transcriptase Polymerase Chain Reaction Language: Persian Journal: Iran. J. Vet. Res. Year: 2008

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Index: IMEMR (Eastern Mediterranean) Main subject: Sensitivity and Specificity / Reverse Transcriptase Polymerase Chain Reaction Language: Persian Journal: Iran. J. Vet. Res. Year: 2008