identification of avian eimeria species in Iran based on biological characteristics

ABSTRACT

One hundred and fourteen litter samples from grand parent, and broiler breeder farms were taken ,according to five different climate areas, to isolate and determine biological characteristics of Eimeria species. 0.5 Kg of each litter samples was used for oocyte isolation. Then, oocytes sporulated at 28' C? for 24-48 hours by oxygenation. The single oocytes were produced and OPG, the time of oocyte excretion and sporulatoin were recorded. Oocytes replicated and isolated from dropping collected on th 4 day after oral inoculation of a single oocytes to 4-6 week chicks. Replicated oocytes of different Eimeria species were ingested by 3-6 week oocyte- free chicks [of necessary quantity] for lesion studies and biometric assay. 25 isolates of oocytes related to different Eimeria species. [E. maxima,E. tenella, E.necatrix, E.acervulina,E.brunette,E.mitis and E.praecox] were categorized based on the following factors Location of lesions in intestine, Gross lesions condition, Oocyte size, shape and color, Schizont and merozoite size, Parasite locationin tissues, Minimum latent period in experimental infection., Minimum required time for sporulation. The resultes were finally confirmed by"Weibridge reference laboratory". The result were analysed by variance analysis was performed by SX statistical Software