Detecting the P24 antigen in lymph nodes of BLV infected cattle

ABSTRACT

In this study 19 BLV infected and 2 healthy lymph nodes were analysed by Western blotting to detect viral p24 antigen. Western blotting was carried out by Rabbit anti p24 serum and p24 monoclonal antibody. Rabbit serum in western blotting could detect 45, 50, 54, 58 and less than 19 kD bands of proteins and monoclonal antibody could detect 30, 32, 39, 45, 50 and 53 kD bands. Our results indicate polymorphism of proteins with p24 epitope in different BLVinfected samples. Otherwise, it could be concluded that BLVstructural proteins sharing p24 epitopes have high degree of phenotypic diversity