Protective effect of vitamin E [alpha tocopherol acetate] on diclofenac-induced nephrotoxicity in young albino rats

ABSTRACT

To determine the preventive role of Vitamin E on renal parenchyma after given of Diclofenac Sodium in young albino rats. Experimental study. This study was carried out in the Department of Anatomy Baqai Medical University and Muhammad Medical College. Mirpurkhas from June 2011 to November 2011. 30 young albino rats were taken. They were divided into three groups A, B and C. The animals in group-A given normal saline 10 ml/kg per day. Group-13 received diclofenac sodium 2 mg/kg per day and group-C receives diclofenac sodium 2mg/kg/day dissolved in distilled water with vitamin-E 2 mg/kg/day dissolved in olive oil administered half an hour before the diclofenac sodium by feeding tube per day for 2 weeks. On day 15 all animals were sacrificed with deep ether anesthesia. Their kidneys were removed, fixed in 10% formalin, Representative blocks were taken and embedded in liquid paraffin. For routine histological examination 5 pm thick section cut by niicrotome and stained with HandE, PAS and silver methenamine Renal histology was done under- light microscope to see the proximal and distal tubular diameter and count. No significant [PT0.05] changes were observed in the histopatholov of kidney tissues of the groups A and C rats. The group B significantly [P<0.001] affected the histopathology of kidney. It may be concluded that diciofenac sodium produces changes in kidne, which may be attributed to ischaemia induced by inhibition of prostaglandin synthesis resulting in tubular necrosis in young albino rats simultaneous administration of vitamin-E partially protect the morphological and histological changes induced by diclofenac sodium