Non-coding CK19 RNA in peripheral blood and tissue of breast cancer patients

ABSTRACT

Breast carcinoma is the major cause of cancer-related death in women. The incidence of this carcinoma is rising and there are many attempts to decrease this problem. The aim of this study was detection of full-length cytokeratin 19 [CK19] mRNA, in peripheral blood and tissue of breast cancer patients in early stage of cancer. In this study, RT-PCR [reverse transcriptase-polymerase chain reaction] technique was used for detection of CK19 mRNA in peripheral blood and tissue of breast cancer patients. Primers were established to amplify the CK19 as a tumor marker. Moreover, CYFRA 21-1 subunit of CK19 protein was measured in the serum of patients. CK19 mRNA was detected and sequenced. It is shown that the most released CK19 mRNAs in blood and tissue of cancer patients are non-coding RNA. The mutated forms of mRNA are the incomplete transcripts of protein-coding gene as a long non-coding RNA [lncRNA] that could regulate gene expression. Moreover, small non-coding RNA [ncRNA] as fragments of CK19 is mostly observed in this experiment. They may play a role in tumorogenesis and their biologic exact function in breast cancer should be further elucidated