Ultra-structural study by transmission electron microscopy: effect of omega-3 on ovary cell organelles after experimental induced polycystic ovary syndrome

ABSTRACT

Polycystic ovary syndrome [PCOS] is the most frequent cause of female infertility, affecting about 4% to 8% of women in the age of procreation. For evaluation the protective effects of omega-3 polyunsaturated fatty acid on ovarian structure in experimental PCO induced by estradiol-valerat, this research was done. Wistar female rats [n=40] were allocated into four groups, one control [n=10] and three test groups [n=30], that one group received omega-3 [60 mg/rat/orally/daily], second and third groups were induced PCO by single injection of estradiol-valerate [16mg/ kg/ i.m], third group also received omega-3 [240 mg/kg] for 60 consequence days. Animals were kept in standard conditions. On day 60, the ovarian tissue of Rats in whole groups were removed and prepared for pathological analysis. Vacuolated area and rough endoplasmic reticulum expanded, de-granulated, disorganized were seen in PCO groups; however, these side effects decreased in the groups that received omega-3 significantly. [p<0.05] in comparison to experiment groups and ovarian weights in PCO experimental decreased significantly [p<0.05]. Results revealed that administration of omega-3 could significantly treat PCO. This suggested that polyunsaturated fatty acid could diminish negative side effects of PCO on ovary tissue