Assessment of alpha4, alphav, beta1 and beta3 integrins expression throughout the implantation window phase in endometrium of a mouse model of polycystic ovarian syndromes
IJRM-Iranian Journal of Reproductive Medicine. 2014; 12 (10): 687-694
in English
| IMEMR
| ID: emr-148984
ABSTRACT
Endometrial integrin expression changes might be a reason for implantation failure in polycystic ovarian syndromes [PCOS]. Assessment of integrin genes and proteins expression upon endometrium in the PCOS experimental mouse model was the main goal of this study. 30 NMRI female mice were equally divided into control, experimental [PCOS; received estradiol valerate [40 mg/kg]] and sham group [received; olive oil]. After 8 weeks, each group was hyper stimulated by 7 IU PMSG and then, after 48hrs, 7 IU HCG was injected. Vaginal plaque was checked. After 5 days, Progesterone and estradiol levels and endometrial tissues were investigated to evaluate of alpha4, alphav, beta1 and beta3 integrins gene and protein by qPCR method and immunohistochemistry, respectively. Tissue samples were assessed and showed that level of progesterone was significantly decreased in PCOS group. Results of molecular part in the amount of alphav, beta3, beta1 and alpha4 gene expressions showed a great difference in beta3 and alphav genes expressions between experimental groups, alphav, beta3, alpha4 and beta1 proteins in the endometrial stroma in the control group were expressed, but they were not detected in PCOS group. According to the results, integrins had different expression patterns in different areas of the endometrium; such as epithelial and stromal. It seems that in PCOS, this pattern has changed and the results might have a great influence on implantation failure. Therefore, this study suggests that a great attention to this problem may be essential in patients who are involved
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Embryo Implantation
/
Gene Expression
/
Integrins
/
Integrin beta1
/
Integrin alpha4
/
Integrin alpha5
/
Integrin beta3
/
Endometrium
/
Mice
Limits:
Animals
Language:
English
Journal:
Iran. J. Reprod. Med.
Year:
2014
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