Effects of light activated bleaching and tetra pack orange juice on micro-morphology and composition of dental enamel

ABSTRACT

To study the effects of 38% Hydrogen peroxide activated with LED light and tetra pack orange juice on micro morphology and mineral content of enamel surface. A total of 45 human enamel discs were incubated in saliva overnight to allow pellicle formation and then divided into three groups 15 for control [G1], 15 for bleaching treatment [G2], and 15 for orange juice immersions [G3]. Surface micro-morphology, calcium and phosphorus were measured with Scanning electron microscope along with energy dispersive spectroscopy five day after treatment. Enamel discs of group 2 were treated with H2O2 with LED and group 3 with orange juice for 15 min each cycle repeated four times for five days to simulate daily treatment with the products for 5 days. The discs were stored in saliva between treatment cycles. Normality of the variables was checked by Shapiro-Wilk's test and all variables were found to be approximately symmetric. Repeated measures ANOVA with post-hoc analysis was run to assess the changes in percentages of calcium and phosphorus due to different application. Mild alteration was seen on enamel surface of control group. The photomicrographs of enamel surface after bleaching and irradiated showed moderate generalized surface roughening. Severe alteration of enamel surface was observed in group 3. Group 3 [Orange juice] produced greater calcium and phosphate loss in comparison with all the other groups [p<0.0001]. No significant difference [p>0.999] was observed in calcium and phosphorus loss between group I [control] and group 2 [bleached]. Group 3 [Orange juice immersion] significantly altered morphology and increased loss of calcium and phosphorus erosion of enamel as compared to group I [control] and group 2 [Bleached] so, there was significant difference between control and immersion group. There was in significant difference between control and bleached group