Nutritional interaction effect of zinc and coffee on serum lipid profile and copper in rats

ABSTRACT

Coffee is one of the most popular beverages in the world. The present work was designed to study the effect of dietary coffee and zinc on serum lipid profile, zinc and copper in serum and liver. Forty two adult male albino rats were divided into six groups and fed on different diets ad libitum for 7 weeks as follows Group [1] fed on standard basal diet, group [2] fed on standard diet supplemented with zinc chloride [ZnCl[2]] at dose of [20 mg/kg diet], groups [3 and 4] fed on standard diet supplemented with boiled coffee at two doses [15 g and 30 g / kg diet] respectively, groups [5 and 6] fed on the previous two diets respectively but in combination with ZnCI[2] [20 mg/kg diet]. At the end of the experiment some biochemical analyses were measured in serum and liver. The results showed significant elevation of serum total lipids [TL], total cholesterol [TC], triacylglycerols [TG], low density lipoprotein-cholesterol [LDL-c], while a significant decrease of high density lipoprotein-cholesterol [HDL-c] in rats fed diet supplemented with low or high dose of coffee. When rats fed zinc with low or high dose of coffee, improvement in lipid parameters were observed. Also serum lipid peroxides as malondialdehyde [MDA] showed significant increase while blood glutathione [GSH] showed significant decrease in rats fed zinc, coffee [low or high dose] or zinc plus coffee [low or high dose]. Diet supplemented with zinc caused significant increase in serum and liver zinc, on the other hand significant decrease in serum and liver copper were recorded. Moreover, the high dose of coffee led to significant decrease in liver zinc. On feeding zinc and coffee together resulted in significant elevation of serum and liver zinc levels, but serum and liver copper decreased significantly. So zinc may be able to overcome the adverse effects of coffee also administration of zinc plus coffee could decrease lipid peroxidation