Variations and genotypes of interleukin-10 in diabetes mellitus complicating chronic hepatitis C virus infection

ABSTRACT

To study whether genetic polymorphism influence lnterleukin-10 [IL-10] production and immune derangement that may contribute to the development of Diabetes Mellitus [DM] in chronic Hepatitis C Virus [HCV] infection, Two groups of HCV positive patients with liver cirrhosis [23 diabetic and 29 non-diabetic] were studied in addition to 10 healthy subjects. IL-10 serum levels were assayed for all the studied groups using ELISA technique. Two single nucleotide polymorphisms [SNPs] in the promoter region of IL-10 gene namely -1082 [A/G] and-592 [A/C] were genotyped in the HCV groups using Polymerase Chain Reaction, restriction fragment length polymorphism [PCR-RFLP] Technique. Serum IL-10 levels were found to be significantly higher in each of HCV groups compared to healthy control group [P<0.01] with positive correlation to liver enzymes, Fasting Blood Sugar [FBS] and negative correlation to serum albumin. Significant differences of IL-10 levels were detected in diabetic compared to non-diabetic HCV patients [9.94 +/- 3.5 versus 7.68 +/- 2.0 pg/ml, P<0.05].The frequency of carriage of allele G of-1082 A/G and allele C of-592 A/C markers were found to be higher in diabetic HCV compared with non diabetic patients [p=0.024, OR=3.12[95% Cl, 1.16- 8.39] and [p=0.045, OR=2.64 [95% Cl, 1.02, 6.84]] respectively. Haplotype analyses of both markers revealed that the carriage of haplotype GC was significantly higher in diabetic HCV compared to that of non diabetic patients [p<0.0001 and OR=7.49] [95% Cl, 3.45, 15.87]] It was concluded that IL-10 gene polymorphism and subsequently high IL-10 levels is associated with chronic HCV infection and may be involved the pathogenesis of diabetes mellitus