Diabetic patients; study of inflammatory markers

ABSTRACT

Inflammation is the one of the major causes for development of type 2 diabetes mellitus and its complications. In this study, association between inflammation and type 2 diabetes mellitus was studied by measuring various inflammatory markers [soluble vascular cell adhesion molecules type - 1, Interleukin - 6 and C- reactive protein] between healthy and diabetic patients. A cross sectional comparative study. The study was conducted at Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi in collaboration with Combined Military Hospital, Rawalpindi and Centre for Research in Experimental and Applied Medicine [CREAM], Army Medical College, Rawalpindi for blood sampling and biochemical assays respectively. The study was performed in 40 human subjects divided into two groups containing 20 subjects each. One group was designated as control while the other was diseased [diabetic] group. Glycemic status was measured to confirm their normal and diabetic state. Inflammatory markers were measured by Enzyme Linked Immunosorbent Assay [ELISA]. Levels of all inflammatory markers [soluble vascular cell adhesion molecules type - 1, Interleukin - 6 and C-reactive protein] were found to be raised in the experimental diabetic groups; 1991.5 +/- 201.97 ng/ml, 24.99 +/- 1.366 pg/ml and 2931 +/- 168.319 respectively compared to the control group; 570.2 +/- 16.526 ng/ml, 6.64 +/- 0.3516 pg/ml and 1806.6 +/- 183.32 respectively. Inflammatory markers were significantly elevated in patients with diabetes mellitus as compared to normal healthy control subjects: