Midstream urine isolates: sensitivity pattern of various antibiotics in pediatric patients

ABSTRACT

The objectives of the study was to determine different microorganisms responsible for causing urinary tract infections UTI in children and to evaluate sensitivity and resistance pattern of different antibiotics used in UTIs. Study was conducted in Children Hospital Complex [CHCM], Multan, Pakistan. Total 125 children having UTI were taken to determine the antibiotic sensitivity and resistance pattern against microorganisms causing urinary tract infections in children. The urine samples were collected in urine bottles and smears were made within 2 hours of sample collection. Urine sample was inoculated on agar media and then incubated for 24 hours. A smear was prepared on a slide form culture of microorganisms and gram testing was conducted. The microorganisms were then characterized by use of API [Analytical Profile Index] MICROBACT TM 24E of Oxoid England. The antimicrobial susceptibility testing of various antibiotics was performed by disc diffusion method. The percentage of Gram +ve bacteria causing urinary tract infections in children was 28%, Gram -ve 62.4% and Yeast 9.6%. The most prevailing species are S. aureus, S. epidermidis and E.coli. Results showed that female children are more prone to UTI than male children. Norfloxacin is effective in S. Aureus UTI while cefaclor is effective in S.epidermidis UTI. Amikacin, Norfloxacin and Cefuroxime are effective in UTI caused by E.coli