Simultaneous determination of liquiritin, nodakenin and glycyrrhizinin Guibi-tang, a traditional herbal prescription by HPLC-PDA

ABSTRACT

A simple, convenient, rapid and accurate high-performance liquid chromatographic [HPLC] method was established for the simultaneous determination about three ingredients of a traditional herbal prescription, Guibi-tang [GBT] liquiritin [1], nodakenin [2] and glycyrrhizin [3]. Chromatographic analysis on the three components was separated within 35 min on a Gemini C[18] column and maintained at 40[degree sign]C. The mobile phase consisted of water with 1.0% [v/v] acetic acid [solvent A] and acetonitrile with 1.0% [v/v] acetic acid [solvent B] in gradient mode at a flow-rate of 1.0 mL/min. Chromatograms were acquired at 254, 280 and 330 nm in a photodiode array [PDA] detector. The calibration curves showed excellent linearity [R[2]=1.0000]. The average recovery of three compounds was >/= 93.5%, with a relative standard deviation [RSD] of