Study of klotho gene transfer for the protective effect of the coronary of diabetic rats

ABSTRACT

The authors model diabetes in rats, and inject the rats with Klotho gene. Then the levels of serum lipoprotein are tested, measuring the thickness of coronary artery intima and the ratio of intima and media to see whether Klotho gene has protective effect of coronary artery in diabetes rats. Extracting the Klotho gene from kidney tissue in the normal SD rats amplifies the target gene by PCR and uses adenovirus as carrier. Then SD rats were randomly divided into model group, control group and the treatment group for diabetes modeling. Transferred the Klotho gene into treatment group and blank adenovirus into control group by the experimenters. Nothing was done for model group. Rats were killed after a successful modeling in the twelfth week, then tested blood low-density lipoprotein, high density lipoprotein, and the coronary artery intima-media thickness. After doing these, intima-media thickness ratio was tested. The high density lipoprotein is 0.67 +/- 0.06 mmol/L in treatment group, 0.48 +/- 0.10mmol/L in control group, 0.47 +/- 0.10mmol/L in model group. The treatment group, control group and model group respectively two independent sample tests. There is statistical significance between treatment group and the other group p<0.01 in treatment group. The low density lipoprotein is 0.44 +/- 0.08 mmol/L in treatment group, 0.45 +/- 0.10mmol/L in control group, 0.44 +/- 0.05mmol/L in model group. Respectively two independent sample test show that there is no statistical significance between treatment group and the other group [p<0.05]. Intima thickness is 1.74 +/- 0.05 micro m in treatment group, 2.23 +/- 0.06 micro m in control group, 2.15 +/- 0.05 micro m in model group. There is statistical significance between treatment group and the other group [p<0.01]. The ratio of intima and media is 0.237 +/- 0.097 in treatment group, 0.308 +/- 0.023 in control group, 0.316 +/- 0.037 in model group, and t test, there is statistical significance between treatment group and the other group [p<0.01]. There is protective effect on coronary after Klotho gene was transferred into diabetic rats