Estimation of glucose in the urine of Diabetic Patients

ABSTRACT

A reduction of Mn [VII] to Mn [VI] in slightly alkaline medium was applied for the detection of total reducing substance [TRS], glucose reducing substances [GRS] and non-glucose reducing substances [NGRS] in the urine of diabetic patients. A determination of GRS in the presence of NGRS in the urine was performed using El-form of the anion exchanger Lewitit MP600. GRS in the urine of uncontrolled diabetic patients ranged between 40 and 3.3 g/l and the range of TRS and NGS was 45-6.6 and 5.0-3.3 g/l, respectively. The concentration of urine reducing substance, which is below or above the limits of Benedict's method, can be determined by the developed method: