Biological and molecular characteristics of zymv isolates from cucumber [cucumis sativus] plant grown in Riyadh, KSA

ABSTRACT

Zucchini yellow mosaic virus[ZYMV] is potyvirus with a worldwide distribution. The virus is the most prevalent in cucurbits. Samples of cucumber plants [Cucumls sativus L.] that show symptoms of virus infection were collected during the 18-May-2009 growing season from field-grown cucumber in Riyadh Saudi Arabia. Based on DAS-ELISA with a polyclonal antiserum against ZYMV and reverse transcription polymerase chain reaction [RT-PCR] analysis using two primer pairs, the potyvirus was identified as strain of ZYMV. The anti-ZYMV antibodies in dot-blot, confirming the identity of the isolated viral particles as a potyvirus immunogenically related to ZYMV. Nucleotides Sequence analysis confirms that the isolated virus belong to potyvirus and its gen bank is GU808330.1. This is the first report on ZYMV as causal virus infecting cucumber in KSA