Interleukin 10 gene promoter polymorphism and risk of diffuse large B cell lymphoma [DLBCL]
Egyptian Journal of Medical Human Genetics [The]. 2014; 15 (1): 7-13
in English
| IMEMR
| ID: emr-154342
ABSTRACT
Given the importance of understanding the genetic variations involved in the pathogenesis of non-Hodgkin's lymphoma [NHL], this work was designed to study the impact of IL-10 [-1082 G/A; rs!800896 and -819 C/T; rs!800871] gene promoter polymorphism on susceptibility of Egyptians to diffuse large B cell lymphoma [DLBCL]; the major type of NHL. To the best of our knowledge, this study is the first one that examines IL-10 promoter polymorphism in DLBCL in Egyptians. Genotyping polymorphism is performed using sequence-specific primers polymerase chain reaction [SSP-PCR] in 100 Egyptian DLBCL patients and 119 normal controls. Circulating plasma levels of IL-10 were measured using Enzyme-linked immunosorbent assay [ELISA]. Insignificant change in IL-10 [-1082 and -819] genotypes was recorded. Although A allele is slightly decreased in DLBCL patients, it did not reach statistical significance. GT haplotype was significantly elevated [P < 0.05] in NHL patients. A significant linkage disequilibrium between the -1082 and 819 SNPs with D' = 0.596 and r[2] = 0.1032 [P < 0.001] was demonstrated. Significantly increased plasma IL-10 [P < 0.01] was found which is positively correlated [r = 0.307; P < 0.01] with the disease Taken together, our findings demonstrated that IL-10 promoter gene polymorphism [-1082 and -819] may not have an influence on the clinical outcome of DLBCL, especially in terms of overall secretion level. Further investigations of other cytokine gene polymorphisms will lead to a better understanding of the disease's biological background:
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymorphism, Genetic
/
Enzyme-Linked Immunosorbent Assay
/
Lymphoma, Large B-Cell, Diffuse
/
Interleukin-10
/
Genotype
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Egypt. J. Med. Hum. Genet.
Year:
2014
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