Cytotoxic activity of vitex trifolia purpurea extracts

ABSTRACT

Defatted 85% crude hot aqueous methanol extract of Vitex trifolia purpurea [AME] successively extracted with, chloroform and ethyl acetate. Cytotoxicity of [AME], chloroform methanol extract [CE], ethyl acetate methanol extract [EE] and the residue obtained from methanol extract after successive extraction [RME] have been evaluated on brine shrimp [Artemia salind] and Hep-G2 cell lines as well. In brine shrimp lethality bioassay the results revealed that the [RME] is the most potent one with LC[50] value 173 microg/ml while LC[50] values of [AME], [CE] and [EE] was 180, 199 and 286 |microg/ml, respectively. As well as the results of cyto-toxic assay against Hep-G2 cell lines are in full agreement with previous results, with IC50 values 6, 10.7, 20.8 and 65.8 microg/ml for [RME], [AME], [CE] and [EE], respectively