Detection of renal brush border membrane enzymes for evaluation of renal injury in neonatal scleredema

ABSTRACT

To evaluate renal brush border membrane enzymes in urine as an indicator for renal injury in neonatal scleredema[NS]. Sixty nine NS patients in our hospital were enrolled and divided into mild group and moderate/severe group. Patients were further randomly divided into therapy and control subgroups for 7 days ligustrazine administration. Urine samples were collected to detect renal brush border membrane enzymes [RBBME] by ELISA and beta[2]-microglobulin [beta[2]-MG] by radioimmunoassay [RIA]. The results were compared with those of 30 normal neonates. Data were statistically analyzed using SPSS13.0 software. Both RBBME and beta[2]-MG were found to be higher in urine in NS patients than normal controls [P < 0.01]. Level of RBBME increased with the severity of NS [P <0.05], while urinary beta[2]-MG did not [P >0.05]. After being treated with ligustrazine, a medicine for renal function recovery, both RBBME and beta[2]-MG were similarly significantly decreased comparing to untreated groups [P < 0.05]. 79.7% of NS patients showed abnormal RBBME while only 52.2% had an abnormal urinary beta[2]-MG [chi[2]=11.65, P < 0.01]. RBBME was more sensitive than beta[2]-MG in reflecting the renal injury in NS. Examination of RBBME effectively reflected the recovery of renal injury after treatment with ligustrazine