Amphotericin B-lnduced in vitro postantifungal effect on candida species of oral origin

ABSTRACT

The aim of this investigation was to measure the Postantifungal effect [PAFE] of 6 different oral Candida species following exposure to amphotericin B. Materials and Methods: Five oral isolates each of Candida albicans, Candida tropicalis, Candida krusel, Candida parapsilosis, Candida gla-brata and Candida guilliermondii [total of 30 isolates] were examined for the presence of PAFE after 1 h of exposure to the minimum inhibitory concentration of amphotericin B. The PAFE was determined as the difference in time [hours] required for the growth of the drug-free control and the drug-exposed test cultures to increase to 0.05 absorbance level following removal of amphotericin B. Results: The mean duration of amphotericin B-induced PAFE was lowest for C albicans [5.91 +/- 0.31 h] and greatest for C. parapsilosis [12.72 +/- 0.11 h], while C. guilliermondii [8.32 +/- 0.33 h], C. glabrata [8.43 +/- 0.21 h], C. krusel [9.68 +/- 0.23 h] and C. tropicalis [10.98 +/- 0.18 h] elicited intermediate values. Conclusion: Even a limited exposure to sublethal concentrations of amphotericin B suppressed growth of Candida species of oral origin. The significant variation in amphotericin B-induced PAFE amongst different Candida species may have clinical implications in terms of amphotericin B regimens used in the management Of Oral Candidiasis