Optimization of secretory expression of recombinant hGM-CSF in high cell density cultivation of recombinant Escherichia coli using Taguchi statistical method
IJB-Iranian Journal of Biotechnology. 2012; 10 (4): 275-280
in English
| IMEMR
| ID: emr-155428
ABSTRACT
Human granulocyte macrophage colony stimulating factor [hGM-CSF] has many therapeutic applications. In this study, in order to verify the purification process, the effect of carbon source, IPTG concentration and post-induction time on the secretion of recombinant hGM-CSF into the culture medium by recombinant Escherichia coli during high cell density cultivation were evaluated by using the Taguchi statistical method. The results indicated that glucose, 1mM IPTG and a time of 6 h post-induction, represented optimum conditions. The secreted hGM-CSF, overall volumetric productivity and purified hGM-CSF were 373 mg/l, 18 mg/l/h and 63 mg/l, respectively
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Macrophage Colony-Stimulating Factor
/
Culture Media, Conditioned
/
Escherichia coli
Language:
English
Journal:
Iran. J. Biotechnol.
Year:
2012
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