Detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria by multiplex polymerase chain reactions
EMHJ-Eastern Mediterranean Health Journal. 1999; 5 (1): 61-70
in English
| IMEMR
| ID: emr-156597
ABSTRACT
The ability of two-band and three-band multiplex polymerase chain reactions to detect and differentiate Mycobacterium tuberculosis complex from non-tuberculous mycobacteria was evaluated. The polymerase chain reactions differentiated between M. tuberculosis and non-tuberculous mycobacteria when standard strains and clinical isolates of mycobacteria were tested. The sensitivity of the two-band and three-band techniques to detect M. tuberculosis in clinical specimens, compared with smear and/or culture, was 88% and 75% respectively. Although both techniques showed 100% specificity, the superior sensitivity of the two-band technique suggests that it could be more useful in the diagnosis of tuberculosis and in differentiating M. tuberculosis complex from non-tuberculous mycobacteria
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Tuberculosis
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Diagnosis, Differential
/
Mycobacterium
/
Mycobacterium Infections
Limits:
Humans
Language:
English
Journal:
East Mediterr Health J.
Year:
1999
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