Radio-immuno and enzyme linked immunosorbent assays for detection of hepatitis A and B antibodies in blood donors sera

ABSTRACT

Hundred paid male blood donors reruited from the blood bank of medical research Institute, Alexandria were tested for anti HBC using ELIZA test, 64 were positive for anti HBC while 89 were positive for anti HA although the routine test for HBS antigen in all those donors was negative. All donors positiveto anti HBC were positive to anti HA while the reverse was not true. Detection of ant1 HA were highest while elder donors be group 0 and A [more younger donors were positive to anti HA while elder donors were positive to anti HBC]. our results denote that vaccination against hepatitis A is of little value as anti HAVAB was detected in high percentage. Also the high percentage of our blood donors should direct our health authorities to take more practical and effective measures to lower hepatitis infection prevalence. This might allow the application for anti HBC test as a routine one in blood banks and reduce transmission of hepatitis B through blood transfusion