Enzyme linked immunosorbent assay [ELISA] for detection of human rota virus in the first two years of life

ABSTRACT

Two hundred infants aged 0-2 years clinically diagnosed as gastroenteritis were tested for the presence if rota virus antigens in their stools by using ELIZA technique. 19% of those infants were positive. The highest antigen detection was in the age group of 6-12 months [30.7%]. No sex difference was observed, most of the antigen detection was in cooler months [autumn and winter 23.6% and 31.2%] as compared with hot months [spring 13.4% and summer 13%]. Twenty one percent if the infants were represented with severe diarrhea, while those with respiratory symptoms account only for 15.7%, but the main bulk of the cases [42.1%] was represented with the triad [diarrhea, vomiting, and fever]. Though rota virus detection in the stool was not that high to be considered as one of the main causes of gastroenteritis in Egypt, yet it reached 30% in the age group of 6-12 monthes