Treatments for B-glucosidase release from yeast cells

ABSTRACT

Among various treatments tested for releasing active beta-glucosidase in a good yield from Kfragilis NRRL Y-1137 cells, sonication was the most efficient method followed in descending order by organic solvents, homogenization breakage and freezing and thawingOptimum activity of the crude beta-glucosidase was obtained at 50C for 10 min at pH 6.2In addition, 0.05 Mof potassium phosphate was the most favorable buffer for beta-glucosidase activity