role of urinary N-acetyl beta-D-glucosaminidase in children with urological problems

ABSTRACT

To assess urinary NAG/ urinary creatinine [NAG/ Cr] ratio in patients with urological abnormalities [normal and abnormal ultrasonography] and compare it with normal healthy children. A prospective study was conducted from November 2012 to April 2013. Urine samples were collected from 70 patients with various urological abnormalities, and from 40 healthy control groups, their age range [1 month-13 years]. Children who were admitted to Emergency Pediatric department, Children Welfare Teaching Hospital / Medical City Complex, Baghdad, Iraq. The Glomerular filtration rate was in normal range in all of them. Urine samples were tested for NAG by [ELISA, Cusabio, China] while, both Serum creatinine [S-Cr] and urine creatinine [U-Cr] were estimated by Jaffe's kinetic method. In this study 29% of the patients had normal ultrasonography and 72% abnormal ultrasonography. The current results revealed that NAG/creatinine index was significantly higher in all patients with [vesicoureteral reflux, hydronephrosis and pyelonephritis] in comparison with cystitis. The assessment of urinary NAG could be considered as a useful marker in prediction of the [vesicoureteral reflux, hydronephrosis] .Urinary NAG is elevated in children with pyelonephritis and it can be considered as a further criterion in the diagnosis of upper urinary tract infection