Histological and biochemical evaluation of the effects of some antioxidants on aged testes

ABSTRACT

Different studies have demonstrated that aging clearly affects male fertility which may be attributed to the androgen deficiency. Reactive oxygen species play a central role in the pathophysiology in the aged-related decrease in male fertility. Some antioxidants have ameliorative effects on different aged organs. The present study aimed to evaluate the effects of some antioxidants on aged testes. Ten adult and fifty aged male albino rats [Rattus albus] were divided into six groups. Group I [control adult], Group II [control aged], Group III [Vitamin E-treated aged], Group IV [Vitamin C-treated aged], Group V [Zinc sulphate-treated aged], Group VI [Vitamin E-, Vitamin C-and Zinc Sulphate-treated aged]. Vitamin E, Vitamin C and Zinc were administrated in doses 2.52 mg, 3.15 mg and 0.693 mg, respectively. Histological and ultrastructural evaluation of the testes were examined as well as Follicle stimulating hormone [FSH], Luteinizing hormone [LH], total and free testosterone levels in the serum were measured. Counting the number of litters per animal and the teratogenic effects was noticed. Giving zinc alone or combined with other antioxidants gave better ameliorative effects on the testicular structure and hormonal levels in the serum. No teratogenic effects of the aged animals`offspring were noticed