Virulence factors in mycoplasma of human origin

ABSTRACT

Although mycoplasmas posses a very limited genome, little is known about their virulence mechanisms and methods of persistence in the host. The significant genome compaction that occurred in mycoplasma was made possible by adopting a parasitic mode of life. Demonstration of the correlation between urogenital infection and some Mycoplasma species. Also detection of some virulence genes of mycoplasma and ureaplasma isolates by PCR. Collection of urine, semen, vaginal swabs and synovial fluid from patients of urogenital manifestation with or without arthritis. Isolation of mycoplasma on the suitable media, identification of the isolates by using different biochemical tests, serological typing of the isolates by specific antisera and detection of some virulence genes by PCR. out of 524 examined samples 84 [16.03%] proved to be positive for mycoplasma by culture. The results revealed that most prevalent Mycoplasma species were M. genitalium [24.74%], M. hominis [23.71%], M. fermentans [38.13%] and U. urealyticum [13.40%] by using specific antisera. PCR technique were used for detection of some virulence gene [16SrRNA, P[1], and P[a] cytoadhesine and gyrA genes] of the isolated mycoplasma and ureaplasma. The results of this study is explained the role of mycoplasma in causing some urogenital infection due to some genes detected, these may responsible for different virulence potentials