Iranian Journal of Cancer Prevention. 2015; 8 (2): 100-108
in English
| IMEMR
| ID: emr-161873
ABSTRACT
Potential association of leptin [LEP] gene polymorphisms has been suggested in the processes leading to breast cancer initiation and progression. We investigated whether genetic variations in the LEP -2548G/A gene are associated with risk of breast cancer. This case-control study consisted of 100 breast cancer cases and 100 control subjects without breast cancer that matched for age and body mass index [BMI]. Genotyping of LEP -2548G/A polymorphism was performed by polymerase chain reaction-restriction fragment length polymorphism [PCR-RFLP] assay. Serum leptin level was determined by ELISA in all study subjects. The genotype distributions [AA, AG, and GG] were 36, 55, and 9% in breast cancer cases and 52, 45, and 3% in control group, respectively. The frequency of LEP -2548 GG genotype was significantly elevated in breast cancer cases as compared to controls [chi2=6.90, p=0.032]. Similar difference was also found in allele frequencies between two groups [chi2=5.65, p=0.017]. A markedly increase risk of breast cancer was associated with the LEP -2548GG genotype when compared to the LEP -2548 AA genotype [OR=4.33, 95% CI=1.09-17.22]. In addition, postmenopausal women who bear at least one LEP -2548 G allele were at a markedly increased risk of breast cancer after adjusting for age and BMI confounders [OR=12.24, 95% CI=1.13-131.73]. The LEP -2548 G/A polymorphism is associated with markedly increased risk of breast cancer especially in postmenopausal Ahvazian women and supported the hypothesis that leptin is involved in breast cancer
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymorphism, Genetic
/
Polymorphism, Restriction Fragment Length
/
Case-Control Studies
/
Polymerase Chain Reaction
/
Leptin
Limits:
Female
/
Humans
Language:
English
Journal:
Iran. J. Cancer Prevention
Year:
2015
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