Comparative studies on the effect of some antioxidants on renal dysfunction in rats

ABSTRACT

The present study is carrying out for investigating the effect of supplementation with some vitamins [A, EandC] as natural antioxidant extracts in renal dysfunction in rates. 40 adult male Sprague - Dawley rats [150 - 200 g] divided to two groups. First group 8 rats were fed on standard diet [S.D.], as a control group. Second group 32 rats were injected intraperitonial with a single dose of Cis-diammine dichloride Platinum II [CDDP] for inducing renal dysfunction [2.5 mg/Kg] then it was divided to six subgroups each one contained 8 rats. [1] fed on [S.D.] nephrotoxic group. [2] fed on [S.D.] + Vitamin A [15 mg/Kg body weight/day]. [3] fed on [S.D.] + Vitamin E [317 I.U. /kg body weight/day]. [4] fed on [S.D.] + Vitamin C [280 I.U. /kg body weight/day]. The experimental period was four weeks, results were statistically analyzed. The results proved that groups of nephrotoxicity rats supplemented with Vitamin A,E and C showed significant increase in food intakes, body weight gain and food efficiency ratio [FER] [28.9%, 29.4% and 19.2%], [870.5%, 1615.6% and 409.8%] and [652.5%, 122502% and 327.3%] respectively, compared with nephrotoxic group. The nephrotoxicity rats supplemented with Vitamin A and showed significant reduction in serum vitamin E and kidney glutathione content [9.3% and 47.9%], while nephrotoxicity rats supplemented with Vitamin E and C showed significant increase in serum vitamin E and kidney glutathione content [27.9% and 116.6%], [13.7% and 55.8%]respectively. The results showed that nephrotoxicity rats supplemented with Vitamin A, E and C showed significant reduction in serum urea nitrogen and creatinine [48.01% and 55.2%], [52.6% and 60.3%] and [57.0% and 63.04%] respectively. Best results in histopathological examination of kidney were in vitamin A and vitamin C groups. These results suggest that natural antioxidants could be beneficial as additional therapy in renal dysfunction