Acute effects of ruta graveolens L. on sperm parameters and DNA integrity in rats

ABSTRACT

Increase in world population is one of the serious and threatening issues in this century. Therefore, it is vitally important to find safe and effective contraceptive methods, especially for men which already have few choices in this regard. Medicinal plants that were used for contraception in ancient times could be good sources of investigation in this filed. Ruta graveolens L. is one the plants introduced in the Iranian traditional medicine as an oral male contraception to be used before intercourse. In this study we tried to investigate the probable effects of the plant on the spermatozoa of male rats. Ruta graveolens L. aqueous extract [5 g/kg] was administered orally to five groups of male rats and sperm motility was checked after half, one, two, four and six hours later. Moreover, one group of rats served as the control group. Subsequently, viability of cells [Eosin-Nigrosin staining], morphological changes [Diff-Quick staining], DNA status [acridine orange dye] and serum testosterone levels were assessed in the treated groups which had significant immotile spermatozoa. For statistical analysis, Student's t-test and one-way ANOVA with Tukey's post-hoc test were employed for comparison between groups. A significant reduction in sperm motility was seen one hour after administration of the extract in the case groups compared to the controls [36% vs. 68.15%, respectively, p<0.01]. The motility gradually increased afterwards, and by 6 hours, it was the same as the control group [65.43% and 68.15%, respectively]. No significant changes were seen in viability, morphology or DNA structure of spermatozoa in each group. Testosterone levels did not show any significant changes in the treated groups when compared with the controls. Since a significant temporary immobility of spermatozoa without any adverse effects on other sperm characteristics occurred upon the administration of Ruta graveolens L. aqueous extract, it seems that this plant might have the potential to be used for the suggested male contraception