QTL mapping of heading date and plant height in Barley cross [Azumamugi]x[Kanto Nakate Gold]

ABSTRACT

To identify quantitative trait loci [QTLs] controlling heading date and plant height, ninety nine F13 recombinant inbred lines [RILs] derived from barley cultivars Azumamugi x Kanto Nakate Gold cross were evaluated. The field trails were conducted at randomized complete block design with two and three replications in 2004 and 2005, respectively. Significant differences and transgrassive segregates were observed among lines for heading date and plant height. Composite interval mapping [CIM] was done based on linkage map constructed using 99 RILs and 100 markers including isozyme, morphological, STS and AFLP markers. A strong QTL controlling 26% phenotypic variation of heading date on chromosome 5HL was located near to the e07m25.3-e12m199.1 markers. The QTL had the same interval of the Sgh[2] locus. Allele inherited from Azumamugi parent in this locus decreased heading date. The QTL for heading date in the map interval of ABC261-ABG055 markers on chromosome 1HL could be identical with the eam8 locus and accounted for 11% of the phenotypic variation. New QTL for plant height was detected near to uzul locus on chromosome 3HL, explaining 52% of the phenotypic variation. The effect of allele transmitted from Azumamugi parent in this locus decreased plant height The QTLs identified on chromosomes 1HL, 3HL and 7HS in relation to earn8, uzul and dspl genes showed pleiotropic effects on controlling heading date and plant height