Comparison between elisa and various stains techniques in laboratory diagnosis of cryptosporidiosis
Journal of the Egyptian Society of Parasitology. 2014; 44 (2): 509-516
in English
| IMEMR
| ID: emr-166032
ABSTRACT
Cryptosporidium spp. is an important parasitic protozoa causing diarrhea which is a severe life-threatening diarrhea especially in immunocompromised hosts. We aimed to evaluate the usefulness of detection of Cryptosporidium spp. copro-antigen from fecal specimens by using enzyme linked immunosorbent assay [ELISA] test and comparing its sensitivity and specificity with some staining methods. The results revealed that Modified Acid-Fast stain is considered better than Giemsa in detecting Cryptosporidium species oocysts in faecal smears as their sensitivity were 67.5% and 53.75% respectively. On contrary, ELISA technique is considered the best method used for detection of cryptosporidial infection as its sensitivity is 90%
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Comparative Study
/
Child
/
Clinical Laboratory Techniques
/
Diarrhea, Infantile
/
Face
/
Hospitals, University
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
J. Egypt. Soc. Parasitol.
Year:
2014
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