In vitro maturation of dromedary camel oocytes in Hamsvs F10 medium supplemented with different concentrations of fetal bovine serum

ABSTRACT

To mature dromedary camel oocytes for using them in an IVF system. Design: Interventional study. Ovaries from dromedary camels in local slaughterhouses. Removing varies from camels in a local slaughterhouse, carrying them to the laboratory in warm saline solution, aspiration of follicles, isolation and transferring of oocytes into TCM-199 and Ham's F10 supplemented with 0-10% heat inactivated fetal bovine serum [FBS], culturing oocytes for up to 24h in a COz incubator. After culture oocytes were denuded and put into PBS containing 0.1% hyaluronidase and passing through a fine pipette. Oocytes were then mounted onto slide glass and fixed and stained for evidence of maturation. ANOVA and when a significance different was seen, Duncan's Multiple Range Test. When oocytes from fresh ovaries were culture in Ham's F10 without protein, only 17.65% of them reached to MIL However, significantly [P<0.05] higher oocytes reached to Mil in 5 and 10% PCS [36.84% and 33.33% for 5 and 10% FBS respectively], which were not dose dependent. When cool stored ovaries were used for oocyte maturation, 14.54% of oocytes reached to MIL In protein-free medium However, significantly [P<0.05] higher oocytes reached to Mil in 5 and 10% PCS [25.86% and 33.33% for 5 and 10% FBS respectively]. Although increasing the protein increased the maturation rates, the difference was not significant. Under the present condition it seems that cool stored ovaries could be used for in vitro maturation of camel oocytes