Cryptosporidia infection and associated bacterial entropathogens in diarrheic calves and cows in dairy farms around Tehran

ABSTRACT

Determination the effects of the bacterialentropathogens to increase infection of calves and cows tocryptosporidium. Descriptive study. A total of 152 calves and 300 cows. Microscopic detection of cryptosporidium inthe direct smears of feces samples from 3 farm in aroundTehran, using smears, fixing with methanol, staining withmodified zeal-nielson, detecting oocysts under a lightmicroscope with objective magnification of 40 X [at leastin 20 microscope field], confirming the observation usingmagnification of 100 X. on the other hand, feces sampleswere cultured for detection of bacterial agents such asSalmonella, E. coli, Proteus, Klebsiella, Campylobacterand Pseudomonas as well as microscopic observation forpresence of acid-fast bacteria in the direct smears of feces. Descriptive statistics.In this study, Cryptosporidium was detected in40.78% of diarrheic calves of which 10.52% of cases hadcryptosporidium alone, and in 30.26% of casescryptosporidium was accompanied with at least one otherpathogenic bacteria. In 3.28% of cases acid-fast organismwas isolated alone. Target pathogens of this study wasisolated at least in 34.33% of feces samples of cows. In28% of cases Cryptosporidium was confirmed and in 5%acid-fast organism was observed. The results of the present study showed thatthe infection rates are significantly higher in calves than incows which may be the due to the age susceptibility toCryptosporidium. It is suggested that hygiene of calf den isof great importance